Flow cytometric analyses of somatic and pollen nuclei in midday flowers (Aizoaceae)
نویسندگان
چکیده
منابع مشابه
Simplifying Complex Flow Cytometric Analyses
Due to the wide variations in fluorescence and light scatter signals produced by different types of cells and particles, researchers using flow cytometry often devote significant time and sample optimizing amplifier gains and PMT voltage settings. It would simplify operation if these settings could be locked down at the factory, but how would the cytometer then capture this wide range of variat...
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Introduction: Loss of inhibitory output from Purkinje cells leads to hyperexcitability of the Deep Cerebellar Nuclei (DCN), which results in cerebellar ataxia. Also, inhibition of small-conductancecalcium-activated potassium (SK) channel increases firing rate f DCN, which could cause cerebellar ataxia. Therefore, SK channel activators can be effective in reducing the symptoms of this disease, ...
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BACKGROUND AND AIMS Understanding the species composition of pollen on pollinators has applications in agriculture, conservation and evolutionary biology. Current identification methods, including morphological analysis, cannot always discriminate taxa at the species level. Recent advances in flow cytometry techniques for pollen grains allow rapid testing of large numbers of pollen grains for D...
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Several methods have been developed to quantify RNA synthesis during the progression of the cell cycle. The rate of RNA synthesis can be detected during different stages of the cell cycle by staining cells with agents that intercalate with nucleic acids. For example, following staining of mammalian cells with acridin orange, the green and red fluorescence that correlates with DNA and RNA conten...
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The distribution of lectin receptors in the human tonsil was studied using 16 biotinylated lectins. The avidin-biotin-peroxidase complex (ABC) method was used on frozen and paraffin-embedded tissue sections. Cell suspensions were also analysed by dual flow cytometry using respective fluorescein isothiocyanate-conjugated lectins and phycoerythrin-labeled anti-CD3 and anti-human immunoglobulin. F...
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ژورنال
عنوان ژورنال: Caryologia
سال: 2016
ISSN: 0008-7114,2165-5391
DOI: 10.1080/00087114.2016.1188359